![]() The first step in the process of manual focus stacking is to take pictures of the selected object or location. The process of manual focus stacking requires two separate steps. Manual focus stacking is an easy and quick photographic process that anyone can learn, regardless of their photographing ability and knowledge. So how do you do manual focus stacking? The process of manual focus stacking may seem complicated and long at first. Manually focus stacking photographs will help you get familiar with your digital editing software.Manually focus stacking photographs will help you learn how to use your specific camera model.Manually focus stacking photographs can help you develop and learn your editing skills.Manually focus stacking photographs will let you practice you photography skills.Manually focus stacking photos allows you to control how your photograph will turn out more than with automatic focus stacking.Manually focus stacked photographs have fine lines.Manually focus stacked photographs are crystal clear.Manually focus stacked photographs have a greater depth of field.Manually focus stacked photographs have more detail.Here are the pros of manual focus stacking Manual focus stacking is an important photography skill that every serious and dedicated photographer should know how to do. In manual focus stacking you can highlight one object to show its importance or beauty by blurring out the other details in different areas of the photograph. As well, another result that is gained from manual focus stacking is the final photograph presenting that selected object or location in a greater depth of field view.Īnother cool thing about manual focus stacking is that you can choose which item in the photograph you want to make center stage. The result of manual focus stacking is a detailed, sharp and crystal clear photograph of a selected object or location. Manual focus stacking involves talking several photos of a frame with a slight variation in focus between them based on objects in the view such as foreground, midground and background and then using post processing to create en enhanced, sharp depth of field not present in the original photos. It can be useful in macro photography, landscape photography, architectural photography and even portrait photography. Manual focus stacking is an important and useful photography technique that any serious and dedicated photographer should know how to do successfully. Read on.Īlthough it may sound like a complicated and unnecessary process, don’t be fooled. Alternative methods include automating the image acquisition process or the stack editing process. As new camera hardware and software became available over time, however, it has become much easier and quicker to accomplish.įor fully manual focusing stacking, you take several photos of a scene varying the focus and then lay them on top of one another using a free or paid photo editing program. Manual focus stacking has been a technique in the world of photography for many years. We will also look at automatic and semi-automatic methods. In this article I will tell you the secrets behind a technique called focus stacking, which is not difficult to do, and how you can perform it manually without fancy expensive gadgets. Look through the list below and pick a program meeting your demands.Ever see those photos that look impossibly sharp? How about ones that have zero blur and are sharp throughout, even when its a macro closeup of an object that would be impossible to capture without a huge amount of light? Also, you can hone compositional skills and learn to work with. First of all, you find out more about the depth of field, aperture, and focus. Learning this technique is very rewarding. With the best focus stacking software in your arsenal, you can get more control over your images. CombineZP - Excellent tool for macro shots.ON1 Photo RAW 2023 - Professional photo organizer and raw processor.Zerene Stacker - Auto-aligns the source images.That’s when such software comes in handy. If you want to keep a particular item in focus, for example, a plant in the foreground, the house in the background, an object in the frame, etc., you have to spend some time and effort to achieve that goal. This depth of field refers to anything from meters to millimeters. Focus stacking software merges several images with different focal points and creates a sharp result.
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![]() To make a left turn, you should turn into:Ģ7. The wheelchair symbol in a parking space means that it is reserved for disabled persons:īut may be used by any driver if no other space is availableīut may be used by any driver for loading and unloadingĢ5. It is illegal to park if a crosswalk is within:Ģ2.ěefore you turn, you must signal continuously for at least:Ģ4. Only when other vehicles can be seen on the roadĢ1. Turn your wheels to the right, toward the edge of the roadĢ0.ěefore you make a turn, use your turn signals: Turn your wheels to the left, away from the edge of the road When you can see the driver in your side mirrorġ9. The driver you just passed signals you over You can see both headlights of the vehicle in your inside rearview mirror It is safe to return to your lane after passing when: Slow and yield the right-of-way to all pedestriansġ8. Stop until the bus is moving or the stop sign arm is no longer extended If you approach a school bus that is picking up or dropping off children, you must: It is illegal to follow fire vehicles traveling in response to an alarm, within:ġ6. Signal your turn as you approach the intersectionġ5. Wait to signal your turn until you are in the intersection If you intend to turn beyond an intersection, you should: Write or read any text-based communicationġ4. Talk to more than one passenger in the vehicle When no speed limit is posted, the maximum speed in a business or residential area is: Turn your wheels to the left, away from the curbġ2. Turn your wheels to the right, toward the curb If you are parking uphill and there is a curb, you should: You should give the right-of-way to any pedestrian who is:Ĩ.ěefore entering a road from an alley or driveway, you must:ġ0. Slow down and permit the emergency vehicle to passĦ. Immediately drive to the right side of the road and stop When you see or hear an emergency vehicle approaching: Stop regardless of your direction of travelģ.Ěll arrived at an uncontrolled intersection at the same time. Stop if you are moving in the same direction of travel The speed limit approaching a school crosswalk is:Ģ.Ě school bus is stopped on a four-lane road with its stop-sign arm extended, you must: More than one answer may be correct choose the best one.ġ. A score of 80% or higher is required to pass the actual examination. Please thoroughly study the Manual before taking the examination. Questions are taken from the Arizona Driver License Manual. The mission of Texas Drive Safe is to provide Texas drivers and teens who will soon be driving with quality programs for a great price.This sample test is designed to help you check your knowledge of the information needed to obtain an Arizona driver license or instruction permit. The Texas driver’s handbook provides important information that every student will need to know in order to drive safely on Texas’ roadways. While the practice test is a useful study aid, it cannot be substituted for careful review of the Texas driver’s handbook. This online practice test was developed and is hosted by I Drive Safely®. Persons who will be applying for a license to operate a motorcycle or commercial vehicle should choose the appropriate category for their needs. The standard model is for persons who will be applying for a license to drive a non-commercial vehicle (e.g. Standard, Motorcycle, or CDL – Three categories of testing are offered. Unlimited testing – You can take the 100-question or the 50-question exam as many times as you like. This is the best option for maximum test preparation. This is a good way to gain an overview of the Texas driver license test questions.ġ00 question test – The 100-question exam repeats questions less often, so you will be exposed to more questions overall. Thanks to Texas Drive Safe, you can do exactly that! We offer an excellent driver license practice test that will familiarize you with the types of questions you’ll be asked on your REAL test.ĥ0 question test – The 50-question test is the basic testing option. What if you could study the kinds of questions you’ll be asked in a simulated testing environment? Wouldn’t that be a great way to prepare for the test? Some test questions can be difficult even if you’ve carefully studied the Texas driver handbook before attempting your road signs and road rules test. When you are ready to take your Texas driver license exam, you definitely don’t want to go in unprepared. Commercial / CDL Take Your Driver License Exam with Confidence! ![]() Not all instructors use the same teaching methods, so choose instructors that are a great match for you. By following your natural body rhythm, you will find that you learn and retain more information, which leads to success. For late risers in high school, try scheduling your most challenging classes for a later time during the day, if the option is available. However, if you are a night owl, you may find that noon may be the time you feel most energetic, so use this as an indicator as to when to start your day. If you are an early bird, be sure to register early morning classes. Most people are night owls, early birds, or somewhere in between. Your peak time is the time of day when all of your cylinders are firing and you feel ready to take on the world. If you are a part-time student and have a more flexible schedule, you can then choose to take summer classes or fewer classes to spread out your work but beware that this may extend your graduation target date.įind the time of day that you have the most energy and use it to your advantage. If you wish to take more, make sure you assess the difficulty of each class before adding it to your baseline. The minimum requirement is often created for you to graduate on time, so use it as a baseline. If you are a full-time student, you may have to take a course load minimum. You may be tempted to fit in as many classes into your timetable as possible, but this can often backfire because you simply do not have enough time to complete all of your work, study, and get adequate rest.Ĭourse load minimums are often a great guide to the right amount of courses to take. In most cases, the phrase the more the better applies, but when it comes to your schedule of courses, this may prove false. See Effective Class Schedule Planning to learn specific tips on high school, college, and grad school schedule planning. Once you have assessed your classes, you may find it may be a better to postpone some of the harder classes and mix in a free elective or two to balance out your schedule. When choosing classes, assess each class and estimate the amount of work it will require. It is important to challenge but not overwhelm yourself during the school year. In college and grad school, there are topics such as Astrophysics that may take some extra study time to grasp key concepts. For example, in high school you can take Honors, AP, or IB classes, which often require extra projects. The level of difficulty can be determined by the complexity of the topic, class assignments, and time needed to study. You should also check with your school counselor or program department for assistance with determining the best path to complete your course load.Īt each educational level, you will still have an opportunity to choose classes to challenge yourself. Once you know your requirements, be sure to schedule enough of these courses to stay on track to graduation. Most schools provide outlines for your requirements in booklets or online. ![]() Whether you are in high school, college, or graduate school, when all of these elements are in balance, you will find that your school year will be more manageable and you will be on track to achieving success.įirst, you need to make sure that you are fulfilling any requirements you need to complete your course of study. Five key factors that can affect your course of study are program requirements, class difficulty, amount of classes, time of day, and instructors. Click on the Print Your Schedule button and you are all done!Ĭreating a class schedule tailored to your needs can make a drastic difference in the quality of your educational experience. Repeat steps one through three for each course in your schedule. To delete a class, click on any empty space in the timeblock. Timeblocks can span several time periods by simply clicking on adjacent blocks. You can add a class to the schedule maker by clicking on the day and time the class starts. The class schedule maker has a weekly calendar template starting with Monday and ending on Friday. With your color-coded timeblocks created, it's time to add them to the timetable. The class schedule maker creates a color-coded timeblock for you. Choose from colors like azure, gray, and coral. ![]() ![]() This schedule generator allows you to choose a class color to keep your timetable organized. It is most helpful to choose a unique name, such as, English 101 and Math 101 to keep track of your timetable. To add your schedule of courses, you must first add your class name. ![]() Plus you can save parts or pages as templates to load in to another page to get you going right away and still edit the layout on them.įor me going with word press and Visual Composer is the best choice and it cost less to. Also after you Finley get it working in wordpress you still then need to edit it in wordpress to add in all you images and media making it a painstaking process i do not think anyone would want to deal with i know i don’t.Ĭomparing the two it comes down to if you want to spend all the extra time designing a layout and then editing it a second time in another program and then uploading it to your website.ĭesign edit and go live all in the same program using wordpress and Visual Composer to make all the same kinds of layout plus a lot more and as you are making the layouts you can add in all your content to have a finished page that can be re-edited at any time making any changes to the content or layout and all be done on a live site. And making it work with wordpress is another story all together as you need to do much more editing to make it even usable in wordpress making you work more and still not ending up with the flexibility of what you would get by using Visual Composer. Now if you need to make a design change to a page done in Responsive Layout Maker pro after it has been posted to a website with all your content in it i am not sure how you would do that with out messing up all the content and everything. Then you still need to upload that finished design to your web server to post as a working page. After your layout is all done you then need to export the layout out of Responsive Layout Maker pro and load it into another program so you can now edit it adding in all you content and photos and so on. You have to design your layout and use place holders as you can not really design a finished page in Responsive Layout Maker pro. So how do you use the Responsive Layout Maker pro ? The stat with the Responsive Layout Maker pro dose not work on a live site in fact you can not even add in images and videos into the layout you are designing you have to add in place holders for what you might add in later using another program. Now lets look at the new Coffee cup Responsive Layout Maker pro You can do everything you need in one place and have all the tools you need to do it with. Visual Composer full layout design and page editing all in one. Lots of real word example sites to view showing how it has been used.choose from 40+ predefined elements divided in logic groups with single click.import and export templates and designs.Extra add ons available yes many of them. ![]()
Barry from Sauquoit, NyOn November 9th 1975, "Love Rollercoaster" by The Ohio Players entered Billboard's Hot Top 100 chart at position #51 and 11 weeks later on January 25th, 1976 it peaked at #1 and spent 16 weeks on the Top 100.īetween 19 the Dayton, Ohio quintet had fourteen Top 100 records two made the Top 10 and both reached #1, the group's other #1 record was "Fire", which reached the top spot for 1 week on February 2nd, 1975.Listen to it, the scream is blood-curdling haha. I hear it now and I still get a chill of horror when I think that's the sound of a girl getting murdered. I was so young when it came out but it horrified me then. Jason from AntarticaThe scream story made this song much more memorable.Kaulynn from TennesseeI had this record when I was young and there were 2 screams and they for sure doesn't match those excuses.Otherwise, all the candy, all the dressing, all the horns, the harmonies, background parts, second guitar parts, third guitar parts, and string parts, Billy, Sugarfoot, and myself would arrange all that stuff on the album." That's where you feel what's going on and how to shake what you're going to shake and move what you're going to move. At that time, funk music came from the bottom. Marshall, of course, played bass guitar, and he was instrumental when we would develop certain grooves, patterns, and stuff like that because the bass and the drums is where the groove really comes from in Black music. Satch was great with coming up with titles and certain things that we would write around musically. We wrote, arranged, and then orchestrated. It was Sugarfoot, Billy Beck, and myself. In a 2020 interview with Okplayer, drummer Jimmy "Diamond" Williams, who first joined the band on their 1974 album Skin Tight, explained how a typical Ohio Players tune came together: "There were basically three people. The ploy worked, as the urban legend gave the song a buzz and helped sell records. Instead of telling the truth, the band agreed to coyly answer "no comment" when asked about the rumor, claiming they took a vow of silence. The scream was actually keyboard player Billy Beck. The band left in the scream as a cruel joke. ![]() She went in the manager's studio while the band was still recording and threatened to sue the band, which is when the manager stabbed her to death. While she was doing it, she found out that the glass was actually fiber glass and the honey reacted with the fiber glass by sticking to her legs and ripping all the skin off. She was kneeling on glass and pouring honey in her mouth. ![]() According to the story, which was started by a DJ in California, this woman was the model who appeared on the album cover. It was rumored that the scream was the sound of a woman being murdered in the studio. During an instrumental break, there is a scream that sounds a little out of place. This song is the subject of an urban myth. ![]() Its direct connect makes it easy and fast to establish a dedicated network connection from US, Europe, Taiwan to mainland China. N2ping is an Internet accelerator designed to meet mainland China web access needs. N2Ping1 là mt ng dng min phí c phát trin bi N2ping, thuc danh mc Công c.Tính ti hin ti ng dng này có hn 1. Tak perlu khawatir, situs Toto88 ini telah memiliki lisensi dan izin resmi dari otoritas berwenang.Whether you are using a computer, smartphone, tablet or TV box, you can connect to the Chinese Internet。 Our app allows you to surf the domestic web without restrictions, without the need for complicated settings.Just download the click and connect.This method reduces your network costs, increases bandwidth throughput, and improves your network connection performance. ![]() N2ping is an Internet accelerator designed to meet mainland China web access needs.You cannot even use an application like WhatsApp or Skype in China. The Chinese firewall blocks all the websites and platforms like Facebook, Google, Twitter, Instagram, etc. N2ping is an Internet accelerator designed to meet mainland China web access needs. N2ping is a performant application accelerator designed to enable and optimize your network connection to China. Especially, Chinese users cannot access any website/app built by a US-based company. Normalmente, los archivos de instalación de este programa tienen los siguientes nombres de fichero: n2ping.exe y UpdateD.exe. Chinese Internet Authorities have censored the internet in such a way that Chinese users cannot any website or application that belongs to non-Chinese companies. The Great Firewall of China is much stronger than the actual Great Wall of China. When we talk about internet restrictions, China is the first country that comes to mind. N2ping is an Internet accelerator designed to meet mainland China web access needs. ![]() ![]() The sides of the portable gazebo have built-in wind and rain panels to help in fighting off any unwanted weather conditions. Finally, after use, fold back in the same fashion in reverse, and place it back in its carry bag for safe storage. Then push up the center hub to secure all framework together, it will be fortified once you hear the clicking sound. After open out each hub, there are 5 in total, which make up each side panel. Open up the packaging and lay it flat on the ground. This camping gazebo assembles in under a minute and can be done alone. With the pavilion and escape models performing the best on the market, Clam is a premium choice with a high sell rate, let’s find out why. An extremely wide range with the quick set escape, escape-XL, traveler, and the popular pavilion models are all available online. Producing portable, lightweight, and fast-pitch gazebos, which are tuned towards camping. We will kick this off with an awesome pop up gazebo for camping from Clam Corporation. Read on to find our reviews of the best cabin tents available to buy on the market.īest Pop Up Camping Gazebos Reviewed 1. This isn’t usually an issue as larger groups tend to camp at campsites with plenty of protection surrounding, if not be sure to find some natural blockades, like a tree line or your car for example. The only drawback is their vulnerability to strong wind as it will smack up against a straight wall (flat surface) quite heavily, unlike with a Dome tent it will glide over aerodynamically. For this reason Cabin tents are top of the list for families, tall ceilings allow for lights, fans and lanterns to be hung without fear of tent poles bending. Because of the straight walls, you get this huge interior space that feels massive compared to a Dome shaped tent that curves inwards towards the centre, losing precious head height. Cabin Tents Cabin tents are straight walled, tall and robust tents that are most ideal for families and big groups, due to their internal space and general sizes starting at 4 person and no lower.We also share why Inflatable tents are considered better than the traditional pole tents in many cases, read on to find out all you need to know on these successful modern tents. We also focus in on some top brands that make the best air tents on the market, such as Quechua, Heimplanet, Coleman, and most notably – Vango. Our best list comprises of the best quality built blow up air tents, that won’t flop within 24 hours. Inflatable Tents Here are our top picks of Inflatable Camping Tents, that really hold up out on the field.Now you know the difference, you can discover out top choices list and also all our detailed reviews on top performing instant tents like the CORE, Coleman, Ozark Trail and many others. Whereas pop ups tend to utilize a spring coil to burst outwards. Unlike the pop up tents that are renowned for being fast pitch, instant tents are much stronger and stable, due to the more robust framework. The frame expands and extends outwards and upwards ‘instantly’ allowing for a fast pitch. Instant tents are tents that come pre-assembled, with a pre-built frame. Instant Tents Here you will find our in-depth reviews on the best instant tents that are available to buy online. ![]() We also have individual reviews on specific models and brands of pop up tents, such as Coleman, Quechua, Gazelle or Ozark trail, in case you wanted more info on the finer details of a particular tent. We have our main lists ranging over all the various types and features, great for those wanting to learn more or just see the best out. Pop Up Tents Here you will find our in-depth reviews on the best pop up tents that are available to buy online.We recommend not using the gazebo in winds above 20mph. We recommended using the tent on a terrain where you can insert the tents pegs. These will help provide stability in windy conditions. Please use the waterproof cover provided in wet conditions. The support poles help strengthen the gazebo and prevent puddles from forming in wet conditions. □□□□ □□ □□□□□□□□: This fantastic design of the ClearSky allows for easy pop up in seconds with the ability to fold down the gazebo into a compact carry case making it the perfect addition for any trip. It is constructed using sturdy transparent PVC creating great heat retention while providing an excellent clear view outside the gazebo. □□□□□□□: Our dome gazebo includes two doors and a heavy-duty zipper allowing free movement through the gazebo. It can also be used to keep plants warm over winter and act as a greenhouse. □□□□□: The ClearSky Transparent Dome Gazebo is great for hot tubs, a range of outdoor furniture, cinema screens its even great for commercial use such as restaurants, events, festivals, weddings and social distancing. ![]() Today there are lots of apps able to bring the idea of time blocking to live. So, is it realistic to utilize this approach for those who manage customer requests? Well, we’d agree and say yes, as even a small amount of control over your schedule will be a helping hand. where you should be reactive to new tasks that can show up at any moment. One of the biggest concerns in regards to time blocking is that it doesn’t work for jobs like customer support, account manager, etc. Time blocking approach will help you to avoid making a choice about what to focus on during the working day and let you have enough time to do the task from start to finish without distraction. Then, get a sketch of your working hours in the calendar view and distribute tasks from most important to least important.Īt the end of each day, review tasks that are not finished as well as any new tasks that have come in and adjust your time blocks for the rest of the week accordingly. ![]() Rather than creating open-ended to-do lists, you have to focus on making a concrete schedule for each day that lays out what types of activities you’re going to do and within which time frame.īriefly, you have to list out things you need to do, prioritize them and estimate how long they will take. Time blocking is a productivity technique based on planning out your days in advance by dedicating specific time blocks to tasks, events, and responsibilities. 3 most common mistakes in time-blocking. ![]() For fans who caught the musician’s Us + Them tour in 2017, there was only a little overlap as Waters seemed to purposely trot out the Floyd material that went overlooked last time as well as some surprises from his solo career. Live Nation is a registered trademark of Live Nation Worldwide, Inc. ROGER WATERS Live Albums (CD, LP, MC, SACD, DVD-A, Digital Media Download) ROGER WATERS Videos (DVD, Blu-ray, VHS etc) 3.21 77 ratings The Wall Live in Berlin 1990 2. Waters played 24 songs over the course of two-and-a-half hours, with a large portion devoted to Pink Floyd material. 40 of Law 7/2017, access to the online dispute resolution platform is available, which is available at the following link: This however, we will decide, in each case, whether or not we adhere to this system, since we believe that conflicts can and should be resolved outside these areas, so We advise that prior to any claim you use our customer service system at Thanks for your understanding. Roger Waters - In The Flesh LIVE FULL CONCERT (SUB ES) Floydian Waters 20.3K subscribers Dislike Share 8,377,150 views LINK TO DOWNLOAD THIS DVD FREE. All tickets for the original date will be honored. This entity has an active conflict resolution policy. EVENT UPDATE: The Roger Waters concert has been rescheduled to August 18, 2022. ![]() ![]() Click on "Privacy Policy and cookies" for more information. For more information you can go to where you will have an exact description of them. Comfortably Numb The Happiest Days of Our Lives Another Brick in the Wall, Part 2 Another Brick in the Wall, Part 3 The Powers That Be The Bravery of Being. Roger Waters Más imágenes Nombre real: George Roger Waters Perfil: English musician, singer-songwriter and composer, born 6 September 1943 in Great Bookham, Surrey, England, United Kingdom. * Ticket prices and availability are subject to change * Live Nation only activates essential cookies on this site in order to use the page correctly. X (output without refer gene id, possible the missing setting of -r) $cuffcompare -R /home/guest/Documents/Coursera_commandline/gencommand_proj4/athal_genes.gtf -o /home/guest/Documents/Coursera_commandline/gencommand_proj4/cufflink_day8/cuffcompare transcripts.gtf Use the same format as above for answers consisting of multiple parts. For this step, using the *.tmap files answer the following,įor both sets. ![]() To consider only the reference transcripts that overlap some input transfrag. Run cuffcompare on the resulting cufflinks transcripts, using the reference gene annotations provided and selecting the option '-R' Question 10: How many multi-exon transcripts were reported for each set? $ grep "exon" transcripts.gtf|cut -f 9|cut -d ' ' -f 2|cut -d '"' -f2|sort|cut -d '.' -f 2 |sort|uniq -d|wc -l $cat transcripts.gtf | grep -v "transcipt" | cut -f9 | grep -v "exon_number \"\""|grep -v "exon_number \"\""|grep "exon_number \"1\""|wc -lĭay16:$ cat transcripts.gtf |grep "exon_number \"2\""|wc -lĭay8:$ cat transcripts.gtf |grep "exon_number \"2\""|wc -l Question 9 : How many single-exon transcripts were in the two sets? $cut -f 1 isoforms.fpkm_tracking |grep "day" |grep -n '2$' Question 8:How many single transcript genes were produced for both sets? $cut -f 1 genes.fpkm_tracking | grep "day"| wc -l Question 6:How many genes were generated by cufflinks for each set (Day8 and Day16)? ![]() Me/guest/Documents/Coursera_commandline/gencommand_proj4/Day16/accepted_hits.bam $cufflinks -o cufflink_day16/ -L day16 -p8 /home/guest/Documents/Coursera_commandline/gencommand_proj4/Day16/accepted_hits.bam Assembling transcripts and estimating abundances. >ğragment Length Distribution: Truncated Gaussian (default) Inspecting reads and determining fragment length distribution. You are using Cufflinks v2.2.1, which is the most recent release. $cufflinks -o cufflink_day8/ -L day8 -p8 /home/guest/Documents/Coursera_commandline/gencommand_proj4/Day8/accepted_hits.bam Same format as above for answers consisting of multiple values or parts. For this portion of the analysis, answer the following questions. Question 5:How many reads were left unmapped from each set?Īssemble the aligned RNA-seq reads into genes and transcripts using cufflinks. $samtools view accepted_hits.bam |cut -f 1,6 | grep "N"|wc -l Question 4:How many spliced alignments were reported for each set? Question 3:How many reads were uniquely aligned in each case? Question 2:How many reads were mapped in each set? Samtools view accepted_hits.bam |more|wc -l Qestion 1 How many alignments were produced for the ‘Day8’ and ‘Day16’ RNA-seq data sets, respectively? Of these: 34 ( 0.1%) have multiple alignments (0 have >20)ĩ9.9% overall read mapping -p 4 -o Day16/ -G athal_genes.gtf -transcriptome-index= athal Day16.fastq Of these: 10 ( 0.0%) have multiple alignments (0 have : 57985 $tophat2 -p 4 -o Day16/ -G athal_genes.gtf -transcriptome-index= athal Day16.fastq $tophat2 -p 4 -o Day8/ -G athal_genes.gtf -transcriptome-index= athal Day8.fastq ![]() If multiple answers are required for one question, separate the answers Include a copy of the reference genome with the name “athal.fa” in the index directory.Īlign both RNA-seq data sets to the reference genome using tophat. NOTE: The genome and annotation data were obtained and modified from the Arabidopsis Information Resources (TAIR) Database,Īnd the RNA-seq reads were extracted from GenBank’s Short Read Archive (SRA).Ĭreate a bowtie index of the genome using bowtie2-build, with the prefix ‘athal’. All files are provided in the archive gencommand_. Your own pipeline are provided in the file “”. Sample command files that you can modify to create Use default parameters unless otherwise specified. The reference genome you will need for the analysis is “athal_chr.fa” and the reference gene annotations are in “Day8.fastq” and “Day16.fastq”), extracted and sequenced the cellular mRNA, and are now set to perform the bioinformaticsĪnalysis. You collected samples at day 8 and day 16 (files In the development of Arabidopsis thaliana shoot apical meristem. You are performing an RNA-seq experiment to determine genes that are differentially expressed at different stages |
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